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Promega pgem-t easy vector ii system
Pgem T Easy Vector Ii System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgem-t+easy+vector+system+ii/pgem+t+easy/pm38038441-251-11-16
Average 90 stars, based on 1 article reviews
pgem-t easy vector ii system - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Characterization of a new simian immunodeficiency virus strain in a naturally infected Pan troglodytes troglodytes chimpanzee with AIDS related symptoms
Article Snippet: The amplified and gel purified products were cloned following the manufacturer's instructions (pGEM-T easy vector system II, Promega, Madison, WI) and ten SIV clones for each time point were sequenced to analyse the viral envelope diversity.

Article Title: Should Cell Salvage Be Used in Liver Resection and Transplantation? A Systematic Review and Meta-analysis
Article Snippet: 420 Results were confirmed by cloning the purified DNA into pGEM-T Easy Vector System II 421 (Promega, cat # A1380), followed by sequencing31.

Article Title: Molecular authentication of three Italian melon accessions by ARMS-PCR and ITS1 (internal transcribed spacer 1) secondary structure prediction
Article Snippet: PCR products were gel-purified and cloned into pGEM-T Easy vector System II (Promega).

Article Title: Within-Host Dynamics of the Hepatitis C Virus Quasispecies Population in HIV-1/HCV Coinfected Patients
Article Snippet: The sequences encompassing the E1/E2 were inserted in the plasmid vector pGEM (pGEM-T Easy Vector System II, Promega Corp., Madison, WI) and transfected into competent Escherichia coli JM109 cells.

Article Title: A Novel Gene SbSI-2 Encoding Nuclear Protein from a Halophyte Confers Abiotic Stress Tolerance in E. coli and Tobacco
Article Snippet: The amplicon was purified from agarose gel and cloned into the pGEM-T Easy vector system II (Promega, Madison, Wisconsin) and sequenced (Macrogen Inc., Seoul, South Korea).

Article Title: Synthetic long oligonucleotides to generate artificial templates for use as positive controls in molecular assays: drug resistance mutations in influenza virus as an example
Article Snippet: To ensure the purity of the standard template, the products were ligated into the pGEM-T Easy Vector System II (Promega, Madison, USA) and transformed into competent Escherichia coli JM109 cells, according to the manufacturer's protocol.

Article Title: Effects of multi-walled carbon nanotubes (MWCNT) under Neisseria meningitidis transformation process
Article Snippet: This fragment was cloned into the pGEM-T Easy Vector System II (Promega Corporation, Madison, WI, USA), to generate the plasmid pLAN6.

Article Title: The IgA of hares ( Lepus sp.) and rabbit confirms that the leporids IgA explosion is old and reveals a new case of trans-species polymorphism.
Article Snippet: PCR products were purified (NucleoSpin Gel and PCR Clean-up kit, Macherey-Nagel, Germany) and cloned into the pGEM-T Easy vector system II (Promega, Madison, WI, USA).

Purification:

Article Title: Characterization of a new simian immunodeficiency virus strain in a naturally infected Pan troglodytes troglodytes chimpanzee with AIDS related symptoms
Article Snippet: The amplified and gel purified products were cloned following the manufacturer's instructions (pGEM-T easy vector system II, Promega, Madison, WI) and ten SIV clones for each time point were sequenced to analyse the viral envelope diversity.

Article Title: Should Cell Salvage Be Used in Liver Resection and Transplantation? A Systematic Review and Meta-analysis
Article Snippet: 420 Results were confirmed by cloning the purified DNA into pGEM-T Easy Vector System II 421 (Promega, cat # A1380), followed by sequencing31.

Article Title: Molecular authentication of three Italian melon accessions by ARMS-PCR and ITS1 (internal transcribed spacer 1) secondary structure prediction
Article Snippet: PCR products were gel-purified and cloned into pGEM-T Easy vector System II (Promega).

Article Title: Within-Host Dynamics of the Hepatitis C Virus Quasispecies Population in HIV-1/HCV Coinfected Patients
Article Snippet: The sequences encompassing the E1/E2 were inserted in the plasmid vector pGEM (pGEM-T Easy Vector System II, Promega Corp., Madison, WI) and transfected into competent Escherichia coli JM109 cells.

Article Title: A Novel Gene SbSI-2 Encoding Nuclear Protein from a Halophyte Confers Abiotic Stress Tolerance in E. coli and Tobacco
Article Snippet: The amplicon was purified from agarose gel and cloned into the pGEM-T Easy vector system II (Promega, Madison, Wisconsin) and sequenced (Macrogen Inc., Seoul, South Korea).

Article Title: Synthetic long oligonucleotides to generate artificial templates for use as positive controls in molecular assays: drug resistance mutations in influenza virus as an example
Article Snippet: To ensure the purity of the standard template, the products were ligated into the pGEM-T Easy Vector System II (Promega, Madison, USA) and transformed into competent Escherichia coli JM109 cells, according to the manufacturer's protocol.

Article Title: Effects of multi-walled carbon nanotubes (MWCNT) under Neisseria meningitidis transformation process
Article Snippet: This fragment was cloned into the pGEM-T Easy Vector System II (Promega Corporation, Madison, WI, USA), to generate the plasmid pLAN6.

Article Title: The IgA of hares ( Lepus sp.) and rabbit confirms that the leporids IgA explosion is old and reveals a new case of trans-species polymorphism.
Article Snippet: PCR products were purified (NucleoSpin Gel and PCR Clean-up kit, Macherey-Nagel, Germany) and cloned into the pGEM-T Easy vector system II (Promega, Madison, WI, USA).

Clone Assay:

Article Title: Characterization of a new simian immunodeficiency virus strain in a naturally infected Pan troglodytes troglodytes chimpanzee with AIDS related symptoms
Article Snippet: The amplified and gel purified products were cloned following the manufacturer's instructions (pGEM-T easy vector system II, Promega, Madison, WI) and ten SIV clones for each time point were sequenced to analyse the viral envelope diversity.

Article Title: Should Cell Salvage Be Used in Liver Resection and Transplantation? A Systematic Review and Meta-analysis
Article Snippet: 420 Results were confirmed by cloning the purified DNA into pGEM-T Easy Vector System II 421 (Promega, cat # A1380), followed by sequencing31.

Article Title: Molecular authentication of three Italian melon accessions by ARMS-PCR and ITS1 (internal transcribed spacer 1) secondary structure prediction
Article Snippet: PCR products were gel-purified and cloned into pGEM-T Easy vector System II (Promega).

Article Title: Within-Host Dynamics of the Hepatitis C Virus Quasispecies Population in HIV-1/HCV Coinfected Patients
Article Snippet: The sequences encompassing the E1/E2 were inserted in the plasmid vector pGEM (pGEM-T Easy Vector System II, Promega Corp., Madison, WI) and transfected into competent Escherichia coli JM109 cells.

Article Title: A Novel Gene SbSI-2 Encoding Nuclear Protein from a Halophyte Confers Abiotic Stress Tolerance in E. coli and Tobacco
Article Snippet: The amplicon was purified from agarose gel and cloned into the pGEM-T Easy vector system II (Promega, Madison, Wisconsin) and sequenced (Macrogen Inc., Seoul, South Korea).

Article Title: Synthetic long oligonucleotides to generate artificial templates for use as positive controls in molecular assays: drug resistance mutations in influenza virus as an example
Article Snippet: To ensure the purity of the standard template, the products were ligated into the pGEM-T Easy Vector System II (Promega, Madison, USA) and transformed into competent Escherichia coli JM109 cells, according to the manufacturer's protocol.

Article Title: Effects of multi-walled carbon nanotubes (MWCNT) under Neisseria meningitidis transformation process
Article Snippet: This fragment was cloned into the pGEM-T Easy Vector System II (Promega Corporation, Madison, WI, USA), to generate the plasmid pLAN6.

Article Title: The IgA of hares ( Lepus sp.) and rabbit confirms that the leporids IgA explosion is old and reveals a new case of trans-species polymorphism.
Article Snippet: PCR products were purified (NucleoSpin Gel and PCR Clean-up kit, Macherey-Nagel, Germany) and cloned into the pGEM-T Easy vector system II (Promega, Madison, WI, USA).

Plasmid Preparation:

Article Title: Characterization of a new simian immunodeficiency virus strain in a naturally infected Pan troglodytes troglodytes chimpanzee with AIDS related symptoms
Article Snippet: The amplified and gel purified products were cloned following the manufacturer's instructions (pGEM-T easy vector system II, Promega, Madison, WI) and ten SIV clones for each time point were sequenced to analyse the viral envelope diversity.

Article Title: Should Cell Salvage Be Used in Liver Resection and Transplantation? A Systematic Review and Meta-analysis
Article Snippet: 420 Results were confirmed by cloning the purified DNA into pGEM-T Easy Vector System II 421 (Promega, cat # A1380), followed by sequencing31.

Article Title: Molecular authentication of three Italian melon accessions by ARMS-PCR and ITS1 (internal transcribed spacer 1) secondary structure prediction
Article Snippet: PCR products were gel-purified and cloned into pGEM-T Easy vector System II (Promega).

Article Title: Within-Host Dynamics of the Hepatitis C Virus Quasispecies Population in HIV-1/HCV Coinfected Patients
Article Snippet: The sequences encompassing the E1/E2 were inserted in the plasmid vector pGEM (pGEM-T Easy Vector System II, Promega Corp., Madison, WI) and transfected into competent Escherichia coli JM109 cells.

Article Title: A Novel Gene SbSI-2 Encoding Nuclear Protein from a Halophyte Confers Abiotic Stress Tolerance in E. coli and Tobacco
Article Snippet: The amplicon was purified from agarose gel and cloned into the pGEM-T Easy vector system II (Promega, Madison, Wisconsin) and sequenced (Macrogen Inc., Seoul, South Korea).

Article Title: Synthetic long oligonucleotides to generate artificial templates for use as positive controls in molecular assays: drug resistance mutations in influenza virus as an example
Article Snippet: To ensure the purity of the standard template, the products were ligated into the pGEM-T Easy Vector System II (Promega, Madison, USA) and transformed into competent Escherichia coli JM109 cells, according to the manufacturer's protocol.

Article Title: Effects of multi-walled carbon nanotubes (MWCNT) under Neisseria meningitidis transformation process
Article Snippet: This fragment was cloned into the pGEM-T Easy Vector System II (Promega Corporation, Madison, WI, USA), to generate the plasmid pLAN6.

Article Title: The IgA of hares ( Lepus sp.) and rabbit confirms that the leporids IgA explosion is old and reveals a new case of trans-species polymorphism.
Article Snippet: PCR products were purified (NucleoSpin Gel and PCR Clean-up kit, Macherey-Nagel, Germany) and cloned into the pGEM-T Easy vector system II (Promega, Madison, WI, USA).

Amplification:

Article Title: Characterization of a new simian immunodeficiency virus strain in a naturally infected Pan troglodytes troglodytes chimpanzee with AIDS related symptoms
Article Snippet: The amplified and gel purified products were cloned following the manufacturer's instructions (pGEM-T easy vector system II, Promega, Madison, WI) and ten SIV clones for each time point were sequenced to analyse the viral envelope diversity.

Article Title: Should Cell Salvage Be Used in Liver Resection and Transplantation? A Systematic Review and Meta-analysis
Article Snippet: 420 Results were confirmed by cloning the purified DNA into pGEM-T Easy Vector System II 421 (Promega, cat # A1380), followed by sequencing31.

Article Title: Molecular authentication of three Italian melon accessions by ARMS-PCR and ITS1 (internal transcribed spacer 1) secondary structure prediction
Article Snippet: PCR products were gel-purified and cloned into pGEM-T Easy vector System II (Promega).

Article Title: Within-Host Dynamics of the Hepatitis C Virus Quasispecies Population in HIV-1/HCV Coinfected Patients
Article Snippet: The sequences encompassing the E1/E2 were inserted in the plasmid vector pGEM (pGEM-T Easy Vector System II, Promega Corp., Madison, WI) and transfected into competent Escherichia coli JM109 cells.

Article Title: A Novel Gene SbSI-2 Encoding Nuclear Protein from a Halophyte Confers Abiotic Stress Tolerance in E. coli and Tobacco
Article Snippet: The amplicon was purified from agarose gel and cloned into the pGEM-T Easy vector system II (Promega, Madison, Wisconsin) and sequenced (Macrogen Inc., Seoul, South Korea).

Article Title: Synthetic long oligonucleotides to generate artificial templates for use as positive controls in molecular assays: drug resistance mutations in influenza virus as an example
Article Snippet: To ensure the purity of the standard template, the products were ligated into the pGEM-T Easy Vector System II (Promega, Madison, USA) and transformed into competent Escherichia coli JM109 cells, according to the manufacturer's protocol.

Article Title: Effects of multi-walled carbon nanotubes (MWCNT) under Neisseria meningitidis transformation process
Article Snippet: This fragment was cloned into the pGEM-T Easy Vector System II (Promega Corporation, Madison, WI, USA), to generate the plasmid pLAN6.

Article Title: The IgA of hares ( Lepus sp.) and rabbit confirms that the leporids IgA explosion is old and reveals a new case of trans-species polymorphism.
Article Snippet: PCR products were purified (NucleoSpin Gel and PCR Clean-up kit, Macherey-Nagel, Germany) and cloned into the pGEM-T Easy vector system II (Promega, Madison, WI, USA).

Agarose Gel Electrophoresis:

Article Title: Characterization of a new simian immunodeficiency virus strain in a naturally infected Pan troglodytes troglodytes chimpanzee with AIDS related symptoms
Article Snippet: The amplified and gel purified products were cloned following the manufacturer's instructions (pGEM-T easy vector system II, Promega, Madison, WI) and ten SIV clones for each time point were sequenced to analyse the viral envelope diversity.

Article Title: Should Cell Salvage Be Used in Liver Resection and Transplantation? A Systematic Review and Meta-analysis
Article Snippet: 420 Results were confirmed by cloning the purified DNA into pGEM-T Easy Vector System II 421 (Promega, cat # A1380), followed by sequencing31.

Article Title: Molecular authentication of three Italian melon accessions by ARMS-PCR and ITS1 (internal transcribed spacer 1) secondary structure prediction
Article Snippet: PCR products were gel-purified and cloned into pGEM-T Easy vector System II (Promega).

Article Title: Within-Host Dynamics of the Hepatitis C Virus Quasispecies Population in HIV-1/HCV Coinfected Patients
Article Snippet: The sequences encompassing the E1/E2 were inserted in the plasmid vector pGEM (pGEM-T Easy Vector System II, Promega Corp., Madison, WI) and transfected into competent Escherichia coli JM109 cells.

Article Title: A Novel Gene SbSI-2 Encoding Nuclear Protein from a Halophyte Confers Abiotic Stress Tolerance in E. coli and Tobacco
Article Snippet: The amplicon was purified from agarose gel and cloned into the pGEM-T Easy vector system II (Promega, Madison, Wisconsin) and sequenced (Macrogen Inc., Seoul, South Korea).

Article Title: Synthetic long oligonucleotides to generate artificial templates for use as positive controls in molecular assays: drug resistance mutations in influenza virus as an example
Article Snippet: To ensure the purity of the standard template, the products were ligated into the pGEM-T Easy Vector System II (Promega, Madison, USA) and transformed into competent Escherichia coli JM109 cells, according to the manufacturer's protocol.

Article Title: Effects of multi-walled carbon nanotubes (MWCNT) under Neisseria meningitidis transformation process
Article Snippet: This fragment was cloned into the pGEM-T Easy Vector System II (Promega Corporation, Madison, WI, USA), to generate the plasmid pLAN6.

Article Title: The IgA of hares ( Lepus sp.) and rabbit confirms that the leporids IgA explosion is old and reveals a new case of trans-species polymorphism.
Article Snippet: PCR products were purified (NucleoSpin Gel and PCR Clean-up kit, Macherey-Nagel, Germany) and cloned into the pGEM-T Easy vector system II (Promega, Madison, WI, USA).

Transformation Assay:

Article Title: Characterization of a new simian immunodeficiency virus strain in a naturally infected Pan troglodytes troglodytes chimpanzee with AIDS related symptoms
Article Snippet: The amplified and gel purified products were cloned following the manufacturer's instructions (pGEM-T easy vector system II, Promega, Madison, WI) and ten SIV clones for each time point were sequenced to analyse the viral envelope diversity.

Article Title: Should Cell Salvage Be Used in Liver Resection and Transplantation? A Systematic Review and Meta-analysis
Article Snippet: 420 Results were confirmed by cloning the purified DNA into pGEM-T Easy Vector System II 421 (Promega, cat # A1380), followed by sequencing31.

Article Title: Molecular authentication of three Italian melon accessions by ARMS-PCR and ITS1 (internal transcribed spacer 1) secondary structure prediction
Article Snippet: PCR products were gel-purified and cloned into pGEM-T Easy vector System II (Promega).

Article Title: Within-Host Dynamics of the Hepatitis C Virus Quasispecies Population in HIV-1/HCV Coinfected Patients
Article Snippet: The sequences encompassing the E1/E2 were inserted in the plasmid vector pGEM (pGEM-T Easy Vector System II, Promega Corp., Madison, WI) and transfected into competent Escherichia coli JM109 cells.

Article Title: A Novel Gene SbSI-2 Encoding Nuclear Protein from a Halophyte Confers Abiotic Stress Tolerance in E. coli and Tobacco
Article Snippet: The amplicon was purified from agarose gel and cloned into the pGEM-T Easy vector system II (Promega, Madison, Wisconsin) and sequenced (Macrogen Inc., Seoul, South Korea).

Article Title: Synthetic long oligonucleotides to generate artificial templates for use as positive controls in molecular assays: drug resistance mutations in influenza virus as an example
Article Snippet: To ensure the purity of the standard template, the products were ligated into the pGEM-T Easy Vector System II (Promega, Madison, USA) and transformed into competent Escherichia coli JM109 cells, according to the manufacturer's protocol.

Article Title: Effects of multi-walled carbon nanotubes (MWCNT) under Neisseria meningitidis transformation process
Article Snippet: This fragment was cloned into the pGEM-T Easy Vector System II (Promega Corporation, Madison, WI, USA), to generate the plasmid pLAN6.

Article Title: The IgA of hares ( Lepus sp.) and rabbit confirms that the leporids IgA explosion is old and reveals a new case of trans-species polymorphism.
Article Snippet: PCR products were purified (NucleoSpin Gel and PCR Clean-up kit, Macherey-Nagel, Germany) and cloned into the pGEM-T Easy vector system II (Promega, Madison, WI, USA).

Mutagenesis:

Article Title: Characterization of a new simian immunodeficiency virus strain in a naturally infected Pan troglodytes troglodytes chimpanzee with AIDS related symptoms
Article Snippet: The amplified and gel purified products were cloned following the manufacturer's instructions (pGEM-T easy vector system II, Promega, Madison, WI) and ten SIV clones for each time point were sequenced to analyse the viral envelope diversity.

Article Title: Should Cell Salvage Be Used in Liver Resection and Transplantation? A Systematic Review and Meta-analysis
Article Snippet: 420 Results were confirmed by cloning the purified DNA into pGEM-T Easy Vector System II 421 (Promega, cat # A1380), followed by sequencing31.

Article Title: Molecular authentication of three Italian melon accessions by ARMS-PCR and ITS1 (internal transcribed spacer 1) secondary structure prediction
Article Snippet: PCR products were gel-purified and cloned into pGEM-T Easy vector System II (Promega).

Article Title: Within-Host Dynamics of the Hepatitis C Virus Quasispecies Population in HIV-1/HCV Coinfected Patients
Article Snippet: The sequences encompassing the E1/E2 were inserted in the plasmid vector pGEM (pGEM-T Easy Vector System II, Promega Corp., Madison, WI) and transfected into competent Escherichia coli JM109 cells.

Article Title: A Novel Gene SbSI-2 Encoding Nuclear Protein from a Halophyte Confers Abiotic Stress Tolerance in E. coli and Tobacco
Article Snippet: The amplicon was purified from agarose gel and cloned into the pGEM-T Easy vector system II (Promega, Madison, Wisconsin) and sequenced (Macrogen Inc., Seoul, South Korea).

Article Title: Synthetic long oligonucleotides to generate artificial templates for use as positive controls in molecular assays: drug resistance mutations in influenza virus as an example
Article Snippet: To ensure the purity of the standard template, the products were ligated into the pGEM-T Easy Vector System II (Promega, Madison, USA) and transformed into competent Escherichia coli JM109 cells, according to the manufacturer's protocol.

Article Title: Effects of multi-walled carbon nanotubes (MWCNT) under Neisseria meningitidis transformation process
Article Snippet: This fragment was cloned into the pGEM-T Easy Vector System II (Promega Corporation, Madison, WI, USA), to generate the plasmid pLAN6.

Article Title: The IgA of hares ( Lepus sp.) and rabbit confirms that the leporids IgA explosion is old and reveals a new case of trans-species polymorphism.
Article Snippet: PCR products were purified (NucleoSpin Gel and PCR Clean-up kit, Macherey-Nagel, Germany) and cloned into the pGEM-T Easy vector system II (Promega, Madison, WI, USA).

Sequencing:

Article Title: Characterization of a new simian immunodeficiency virus strain in a naturally infected Pan troglodytes troglodytes chimpanzee with AIDS related symptoms
Article Snippet: The amplified and gel purified products were cloned following the manufacturer's instructions (pGEM-T easy vector system II, Promega, Madison, WI) and ten SIV clones for each time point were sequenced to analyse the viral envelope diversity.

Article Title: Should Cell Salvage Be Used in Liver Resection and Transplantation? A Systematic Review and Meta-analysis
Article Snippet: 420 Results were confirmed by cloning the purified DNA into pGEM-T Easy Vector System II 421 (Promega, cat # A1380), followed by sequencing31.

Article Title: Molecular authentication of three Italian melon accessions by ARMS-PCR and ITS1 (internal transcribed spacer 1) secondary structure prediction
Article Snippet: PCR products were gel-purified and cloned into pGEM-T Easy vector System II (Promega).

Article Title: Within-Host Dynamics of the Hepatitis C Virus Quasispecies Population in HIV-1/HCV Coinfected Patients
Article Snippet: The sequences encompassing the E1/E2 were inserted in the plasmid vector pGEM (pGEM-T Easy Vector System II, Promega Corp., Madison, WI) and transfected into competent Escherichia coli JM109 cells.

Article Title: A Novel Gene SbSI-2 Encoding Nuclear Protein from a Halophyte Confers Abiotic Stress Tolerance in E. coli and Tobacco
Article Snippet: The amplicon was purified from agarose gel and cloned into the pGEM-T Easy vector system II (Promega, Madison, Wisconsin) and sequenced (Macrogen Inc., Seoul, South Korea).

Article Title: Synthetic long oligonucleotides to generate artificial templates for use as positive controls in molecular assays: drug resistance mutations in influenza virus as an example
Article Snippet: To ensure the purity of the standard template, the products were ligated into the pGEM-T Easy Vector System II (Promega, Madison, USA) and transformed into competent Escherichia coli JM109 cells, according to the manufacturer's protocol.

Article Title: Effects of multi-walled carbon nanotubes (MWCNT) under Neisseria meningitidis transformation process
Article Snippet: This fragment was cloned into the pGEM-T Easy Vector System II (Promega Corporation, Madison, WI, USA), to generate the plasmid pLAN6.

Article Title: The IgA of hares ( Lepus sp.) and rabbit confirms that the leporids IgA explosion is old and reveals a new case of trans-species polymorphism.
Article Snippet: PCR products were purified (NucleoSpin Gel and PCR Clean-up kit, Macherey-Nagel, Germany) and cloned into the pGEM-T Easy vector system II (Promega, Madison, WI, USA).

Transfection:

Article Title: Characterization of a new simian immunodeficiency virus strain in a naturally infected Pan troglodytes troglodytes chimpanzee with AIDS related symptoms
Article Snippet: The amplified and gel purified products were cloned following the manufacturer's instructions (pGEM-T easy vector system II, Promega, Madison, WI) and ten SIV clones for each time point were sequenced to analyse the viral envelope diversity.

Article Title: Should Cell Salvage Be Used in Liver Resection and Transplantation? A Systematic Review and Meta-analysis
Article Snippet: 420 Results were confirmed by cloning the purified DNA into pGEM-T Easy Vector System II 421 (Promega, cat # A1380), followed by sequencing31.

Article Title: Molecular authentication of three Italian melon accessions by ARMS-PCR and ITS1 (internal transcribed spacer 1) secondary structure prediction
Article Snippet: PCR products were gel-purified and cloned into pGEM-T Easy vector System II (Promega).

Article Title: Within-Host Dynamics of the Hepatitis C Virus Quasispecies Population in HIV-1/HCV Coinfected Patients
Article Snippet: The sequences encompassing the E1/E2 were inserted in the plasmid vector pGEM (pGEM-T Easy Vector System II, Promega Corp., Madison, WI) and transfected into competent Escherichia coli JM109 cells.

Article Title: A Novel Gene SbSI-2 Encoding Nuclear Protein from a Halophyte Confers Abiotic Stress Tolerance in E. coli and Tobacco
Article Snippet: The amplicon was purified from agarose gel and cloned into the pGEM-T Easy vector system II (Promega, Madison, Wisconsin) and sequenced (Macrogen Inc., Seoul, South Korea).

Article Title: Synthetic long oligonucleotides to generate artificial templates for use as positive controls in molecular assays: drug resistance mutations in influenza virus as an example
Article Snippet: To ensure the purity of the standard template, the products were ligated into the pGEM-T Easy Vector System II (Promega, Madison, USA) and transformed into competent Escherichia coli JM109 cells, according to the manufacturer's protocol.

Article Title: Effects of multi-walled carbon nanotubes (MWCNT) under Neisseria meningitidis transformation process
Article Snippet: This fragment was cloned into the pGEM-T Easy Vector System II (Promega Corporation, Madison, WI, USA), to generate the plasmid pLAN6.

Article Title: The IgA of hares ( Lepus sp.) and rabbit confirms that the leporids IgA explosion is old and reveals a new case of trans-species polymorphism.
Article Snippet: PCR products were purified (NucleoSpin Gel and PCR Clean-up kit, Macherey-Nagel, Germany) and cloned into the pGEM-T Easy vector system II (Promega, Madison, WI, USA).



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